Journal: Frontiers in Cell and Developmental Biology

Article Title: The Lack of a Representative Tendinopathy Model Hampers Fundamental Mesenchymal Stem Cell Research

doi: 10.3389/fcell.2021.651164

Figure Lengend Snippet: Overview of bioreactors used in tendon tissue engineering which all apply mechanical stimulation.

Article Snippet: , , Collagen type I gel + avian tenocytes , Tissue Train 3D Culture System (Flexcell International), culture plate with 2 anchors , 1 h/day, 1% elongation, 1 Hz, 11 days , Tenogenic gene expression and linear morphology. Stronger loaded constructs vs. non-exercised controls..

Techniques: Construct, Expressing, Cell Function Assay, Incubation, Gene Expression, Activity Assay, Control

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Morphology of ADMSCs grown inside a 3D matrix prepared from platelet-rich plasma (PRP) ( a1 , a2 , a3 ; 100×) or PPP ( b1 , b2 , b3 ; 100×), at different time points (0, 72, 144 h). The initial round-shaped morphology (a1, b1; time = 0 h), is changed in a spindle, fibroblast-like morphology at 72 and 144 h ( a2 , b2 , a3 , and b3 ). Cells gradually populate the whole 3D fibrin-derived scaffold, spreading over the entire thickness. No morphological differences are observed between cells maintained in PRP or platelet-poor plasma (PPP) derived matrix.

Article Snippet: Average Doubling Time ± SD , PRP 3D matrix , 26.2 ± 4.6 , 27.8 ± 5.1.

Techniques: Clinical Proteomics, Derivative Assay

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Average cell number, doubling time and cell doubling number obtained at different time points (72, 144 h) from ADMSCs cultured inside a 3D fibrin-based matrix prepared from PRP or PPP (n = 3).

Article Snippet: Average Doubling Time ± SD , PRP 3D matrix , 26.2 ± 4.6 , 27.8 ± 5.1.

Techniques: Cell Culture

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Box plot illustrating differences in average cell number ( a ), cell doubling time ( b ), and cell doubling number ( c ) of cells maintained inside a 3D matrix prepared from PRP or PPP, at different time points (72, 144 h) (n = 3). No statistically significant differences are observed between the two culture conditions.

Article Snippet: Average Doubling Time ± SD , PRP 3D matrix , 26.2 ± 4.6 , 27.8 ± 5.1.

Techniques:

Journal: Bioengineering

Article Title: Role of Collagen in Airway Mechanics

doi: 10.3390/bioengineering8010013

Figure Lengend Snippet: Airway disease stiffness alteration and associated collagen change.

Article Snippet: Aging lung , Increased collagen and decreased elastin production by fibroblasts , 3D matrix model and lung tissue , Increased pulmonary stiffness Decreased compliance , Collagen fibers have higher stiffness and lower deformation.

Techniques: Mutagenesis, Control, Expressing, Membrane, Generated, Tomography, Synthesized

Journal: Cells

Article Title: iPSCs as a Platform for Disease Modeling, Drug Screening, and Personalized Therapy in Muscular Dystrophies

doi: 10.3390/cells8010020

Figure Lengend Snippet: List of key studies using Induced pluripotent stem cells (iPSCs) for disease modeling in case of muscular dystrophies.

Article Snippet: DMD/LGMD BMD , iPSC-human , 3D matrix differentiation to observe myofibers formation. Triple lineage constructs created with 70% myogenic cells and 30% vascular , Development of 3D hydrogel platform for muscle stem cell and myofibers formation , 2018 , Maffioletti et al. [ ] .

Techniques: Retroviral, Transduction, In Vivo, Injection, Functional Assay, Expressing, Transplantation Assay, Inhibition, Transfection, Plasmid Preparation, Marker, Generated, Patch Clamp, Fluorescence, Construct, Cell Culture

Journal: Cells

Article Title: iPSCs as a Platform for Disease Modeling, Drug Screening, and Personalized Therapy in Muscular Dystrophies

doi: 10.3390/cells8010020

Figure Lengend Snippet: List of key studies using iPSCs for gene correction in case of muscular dystrophies.

Article Snippet: DMD/LGMD BMD , iPSC-human , 3D matrix differentiation to observe myofibers formation. Triple lineage constructs created with 70% myogenic cells and 30% vascular , Development of 3D hydrogel platform for muscle stem cell and myofibers formation , 2018 , Maffioletti et al. [ ] .

Techniques: HAC Assay, Sequencing, Transfection, Expressing, Functional Assay, CRISPR, Electroporation, Knock-In, Mutagenesis

Journal: Nature reviews. Cancer

Article Title: Cancer cell motility: lessons from migration in confined spaces

doi: 10.1038/nrc.2016.123

Figure Lengend Snippet: Summary of confined cell migration modes

Article Snippet: Lobopodial , Adhesions required , High RHOA levels , Linearly elastic 3D matrix , Nonpolarized cortactin, PIP 3 , RAC1 and CDC42 , Efficient migration following CDC42 or RAC1 knockdown , 64,80.

Techniques: Migration, Pore Size, Inhibition, Knockdown, Activation Assay